rabbit anti-mouse cd206  (Thermo Fisher)

Journal: Frontiers in Immunology

Article Title: The role of the C5a-C5aR pathway in iron metabolism and gastric cancer progression

doi: 10.3389/fimmu.2024.1522181

Figure Lengend Snippet: C5a-C5aR pathway promoted macrophages polarization to M2 phenotype in vitro . THP-1 cells were cultured in 6-well plates, with 1×10 6 cells per well, pre-treated with 100 nM phorbol 12-myristate 13-acetate (PMA) for 24h. While RAW264.7 cells were cultured in 6-well plates, with 5×10 5 cells per well. Then both cell types were stimulated with 100 ng/mL C5a for 48 hours. In certain experiments, cells were pretreated with 10 nM C5aRA for 3 hours prior to C5a stimulation. After the 48-hour incubation period, cell samples were harvested for quantitative RT-PCR analysis. (A) Relative gene expression of CCR7 and CD206 in THP-1 cell treated with different group (Control, C5a or C5a+C5aRA) were examined by QRT-PCR (n=3). (B) Relative gene expression of CCL3 and CD206 in RAW264.7 cell treated with different group (Control, C5a or C5a+C5aRA) were examined by QRT-PCR (n=3). Statistical analyses were executed with one-way ANOVA test. Data are presented as mean ± standard deviation (SD). Significance levels were set at p<0.01 (denoted by **), p<0.001 (denoted by ***), and p<0.0001 (denoted by ****).

Article Snippet: For multiplex immunofluorescence, the expression of CD206 in mice tumor tissues was detected with a monoclonal rabbit anti-mouse CD206 antibody (1:200, Proteintech, Cat#18704-1-AP).

Techniques: In Vitro, Cell Culture, Incubation, Quantitative RT-PCR, Expressing, Control, Standard Deviation

Journal: Cardiovascular Diabetology

Article Title: Lipoxin A 4 improves cardiac remodeling and function in diabetes-associated cardiac dysfunction

doi: 10.1186/s12933-024-02501-x

Figure Lengend Snippet: Effects of lipoxin A 4 on macrophage polarization in LV. A Representative images of macrophages in the left ventricle, co-stained with nuclei (blue, DAPI) and macrophage (red, CD68). Scale bar = 200 μm. B Quantitative data of CD68-positive macrophages. C Representative images of M1-like macrophages in the left ventricle, co-stained with nuclei (cyan, DAPI) and M1-like macrophage (magenta, iNOS). Scale bar = 200 μm. D Quantitative data of iNOS-positive macrophages. E Representative images of M2-like macrophages in the left ventricle, co-stained with nuclei (cyan, DAPI) and M2-like macrophage (grey, CD206). Scale bar = 200 μm. F Quantitative data of CD206-positive macrophages. G M1/M2 macrophage ratio H mRNA expression of mCd86 in mouse left ventricle. I mRNA expression of mArg1 in mouse left ventricle. Data are presented as mean ± SEM, Two-way ANOVA followed by Fisher’s LSD post hoc test was used to compare the effects of phenotype and treatment. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. Arg1 , arginase-1

Article Snippet: Slides were incubated with either rat anti-mouse CD68 + (1:200, Bio-Rad, Hercules, California, USA), rat anti-mouse iNOS (1:100, Thermo Fisher Scientific™, Melbourne, Victoria, Australia), or rabbit anti-mouse CD206 + (1:100, Thermo Fisher Scientific™, Melbourne, Victoria, Australia), and then placed into a humidified chamber and incubated at 4 °C overnight.

Techniques: Staining, Expressing

Journal: Microorganisms

Article Title: Streptococcus suis Induces Macrophage M1 Polarization and Pyroptosis

doi: 10.3390/microorganisms12091879

Figure Lengend Snippet: Protocols for confocal microscopy.

Article Snippet: To determine the type of macrophage polarization in thymus tissue after bacterial infection, sections from infected mice (2 dpi) were stained with different antibodies, including rabbit anti-mouse CD206 (ABclonal, Beijing, China) or rabbit anti-mouse interleukin (IL)-1β (ABclonal).

Techniques: Microscopy

Journal: Microorganisms

Article Title: Streptococcus suis Induces Macrophage M1 Polarization and Pyroptosis

doi: 10.3390/microorganisms12091879

Figure Lengend Snippet: Thymic macrophage polarization in S. suis infection. Using confocal laser scanning microscopy, appropriate FITC-conjugated antibodies were used to label cells in sections from thymus of infected mice; M1 macrophage marker IL-1β (green); M2 macrophage marker CD206 (green). Macrophages were stained with F4/80 antibody (red) and cell nuclei (blue) were stained with DAPI; BF: Bright-field. Scale bars, 5 μm.

Article Snippet: To determine the type of macrophage polarization in thymus tissue after bacterial infection, sections from infected mice (2 dpi) were stained with different antibodies, including rabbit anti-mouse CD206 (ABclonal, Beijing, China) or rabbit anti-mouse interleukin (IL)-1β (ABclonal).

Techniques: Infection, Confocal Laser Scanning Microscopy, Marker, Staining